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ganS [2018-05-04 11:42:42]
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ganS [2018-05-04 11:42:42]

galactotriose ABC transporter, binding protein
locus
BSU34160
pI
5.44
mw
45.38 kDa
protein length
421 aa Sequence Blast
gene length
1263 bp Sequence Blast
function
uptake of galactotriose
product
galactotriose ABC transporter, binding protein
essential
no
synonyms
yvfK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.1|Uptake of carbon sources]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.24|Utilization of galactan]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,507,253 → 3,508,518

    Phenotypes of a mutant

  • a ''[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]'' mutant is impaired in the utilization of galactan [Pubmed|22893383]

    • The protein

    Protein family

  • bacterial solute-binding protein 1 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|5771E1976D986638B13ADDE97EDE32709E355923|MdxE]

    • [SW|Localization]

  • cell membrane (via [protein|75B1FD4AA7612B3B600CB0CE35319A6960536095|GanP]-[protein|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|GanQ]) [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]
    description
    [Pubmed|9287030]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|27501980], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|1F787F70C87DEB221709579122D63C2322A84E56|GanR]: repression, [Pubmed|9287030], in [regulon|1F787F70C87DEB221709579122D63C2322A84E56|GanR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [pubmed|28617843], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • induced by galactan (binding of galactobiose to [protein|1F787F70C87DEB221709579122D63C2322A84E56|GanR]) [Pubmed|28617843,27501980]
  • repressed by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|28617843,27501980]
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    Biological materials

    Mutant

  • MGNA-A484 (yvfK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/484 NBRP B. subtilis, Japan]
  • BKE34160 (Δ[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATTGTCACCCTTTCG, downstream forward: _UP4_TAAAGAGAAAGCTAGATTTG
  • BKK34160 (Δ[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATTGTCACCCTTTCG, downstream forward: _UP4_TAAAGAGAAAGCTAGATTTG

    • References

  • 11682178,10092453,9287030,22893383,27501980